Huh7 is a human hepatocellular carcinoma cell line, was established by Naka-bayashi et al, the cell from a Japanese male high differentiated hepatocellular carcinoma, HBV negative, can produce some cytoplasmic proteins, such as albumin, a antitrypsin, AFP etc.Huh7 cell lines: they are polygonal at low densities and tend to be spindle shaped when they are dense.